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Mutant huntingtin does not induce binding of HSF1 to HSE. A, HSF1 EMSA analysis of lysates from cells incubated at 43 °C for 1 h or treated with 3 μm celastrol or 10 μm MG132 for 15 h. B, HSF1 EMSA analysis of lysates from cells transiently transfected with HTT(Qn)-mCherry for 72, 96, or 120 h. C, HSF1 EMSA analysis (left-hand panel) of lysates from cells transiently transfected with HTT(Q91)-mCherry and sorted according to low or high mCherry expression levels (right-hand panel).
