FIGURE 4.

Rap1 binding interface for KRIT1 is highly homologous to HRas. A, summary of the KRIT1-Rap1 interactions as identified using PDBePISA. The upper section shows the hydrogen bonds between the two proteins, and the lower section shows the residues buried by formation of the complex. HRas is very similar to Rap1, and the residues that are different are highlighted. B, view of the KRIT1 F1 and F2 subdomains on the surface of Rap1. Both the F1 and F2 subdomains make contact with Rap1. C and D, surface electrostatic potential as follows: C, Rap1; and D, HRas (PDB 1lfd). The dotted line separates the binding interface for KRIT1 F1 and F2 subdomains as shown in B.