FIGURE 1.

The involvement of IL-32β in IL-10 production in U937 cells. A, IL-32β was knocked down in U937 cells by transfection with 1.5 μg of IL-32 siRNA or nontargeting siRNA (NT), and the cells were then treated with 10 nm PMA for 24 h. The culture media were collected for ELISA, and cell lysates were prepared for Western blotting. con, control. The silencing of IL-32β was confirmed by Western blotting. B–D, ELISAs for IL-10 (B), TNF-α (C), and IL-8 (D) were performed. All values are mean ± S.E. (n = 3). *, p < 0.03 (B). siIL-32, IL-32 siRNA; siCtrl, siRNA control.