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PMA-dependent shift of IL-32β localization in U937 cells. Immunofluorescence staining was performed to localize the endogenous IL-32β in U937 cells. Cells were treated with 50 nm PMA or 1 μg/ml LPS for 40 min. Cells were fixed, permeabilized, and labeled for nuclei with DAPI and endogenous IL-32 with FITC. Fluorescence signals were analyzed by confocal microscopy. The same amount of mock IgG (1 μg) as KU32-52 antibody was used for control.
