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. 2013 Jun 30;288(33):23650–23658. doi: 10.1074/jbc.M113.465575

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — The suppression of C/EBPα binding to IL-10 promoter by IL-32β. ChIP was performed with U937 cells after silencing of IL-32β. U937 cells were transfected with 1.5 μg of IL-32 siRNA and nontargeting siRNA (NT). After an overnight incubation following transfection, 10 nm PMA was treated for 90 min. ChIP was carried out with 3 μg of C/EBPα antibody. Quantitation is shown below the PCR data, normalized to control (con) nontargeting siRNA for the others. The consensus binding sequence of C/EBP and a putative C/EBPα-binding site and its location on IL-10 promoter are presented. ChIP results from Bcl-2 and lactoferrin promoters are presented as controls. IL-10 ChIP products were also measured by quantitative real-time PCR. The results are given as -fold change of the IL-10/lactoferrin ChIP ratio as compared with the value of nontreated nontargeting siRNA. All values are mean ± S.E. (n = 3).