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. 2013 Jun 30;288(33):23650–23658. doi: 10.1074/jbc.M113.465575

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Interaction domain mapping of C/EBPα and IL-32β. A, functional domains of C/EBPα and its deletion mutants are schematically illustrated. TAD; transactivation domain, RD; regulatory domain, DBD; DNA-binding domain, bLZ; basic leucine zipper domain. HEK293 cells were co-transfected with 6×Myc-tagged C/EBPα mutants and 5×FLAG-tagged IL-32β. After overnight incubation, cells were treated with 20 nm PMA for 90 min. Immunoprecipitation was performed with 1 μg of FLAG antibody. B, a schematic illustration of IL-32β deletion mutants is presented. HEK293 cells were co-transfected with 5×FLAG-tagged C/EBPα and 6×Myc-tagged IL-32β mutants. Immunoprecipitation was performed as in A with 1 μg of FLAG antibody. The pulled down D1 is marked by arrow. The expression levels of the transfected genes were determined by Western blotting with 30 μg of whole cell lysates (WCL). Immunoglobulin G light chain (IgG L) is indicated by an arrow.