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. 2013 Jul 8;288(33):23680–23686. doi: 10.1074/jbc.M113.480186

FIGURE 1.

FIGURE 1.

MIC-1 expression is increased by RNPC1a. A–C, MCF7, RKO, and H1299 cells were induced or uninduced with 0.5 μg/ml tetracycline to express RNPC1a for 24, 48, and 72 h. The levels of RNPC1a, MIC-1, p21, p53, actin, and GAPDH were measured by Western blotting. D, MCF7 cells were induced or uninduced with tetracycline for 48 h. Levels of RNPC1a, p21, pro-MIC-1, and actin were analyzed through Western blotting. Secreted MIC-1 was detected by Western blotting after immunoprecipitation (IP) with conditioned medium gathered from induced or uninduced MCF7 cells. IB, immunoblotting. E, levels of RNPC1a, MIC-1, and GAPDH mRNA were analyzed by RT-PCR. RNA was isolated from cell extracts obtained from MCF7, RKO, H1299, and HCT116 cells that were uninduced or induced to express RNPC1a for 36 h. preMIC-1, premature MIC-1 mRNA.