FIGURE 5.

MIC-1 knockdown reduces RNPC1-induced cell growth suppression. A, RKO cells were uninduced or induced to express RNPC1a for 48 h. Cell lysates were collected and analyzed by Western blotting. B, colony formation assay was performed using the same cells and treatment as in A. The assay was done in triplicate, and a representative image is shown. C, quantified data were obtained from colony formation plates. Cell density for uninduced cell line RKO-RNPC1a clone 17 was set at 1 as a reference point. *, p = 0.005. Error bars, S.D. D, a model shows RNPC1 regulation of MIC-1 in response to a stress signal.