FIGURE 6.

Electrophoretic mobility shift assays for the DNA binding activity of Ms6564 on different DNA substrates. A, DNA substrates designed for the DNA binding activity assays. The bases bound by Ms6564-K47 (indicated by magenta arrows) or Ms6564-Q48 (indicated by blue arrows) in the crystal structure are highlighted. B, EMSA assays for DNA binding activity of Ms6564 on different DNA substrates. ³²P-Labeled DNA substrates were co-incubated with increasing amount (1.4–7 μm) of Ms6564 and loaded onto 5% polyacrylamide/bis (37.5:1) gels and run at a constant voltage of 100 V. The concentrations of the proteins are indicated.