FIGURE 2.

Activation of a genetic program that controls eicosanoid metabolic pathway and signaling in unelicited NPC2-fibroblasts; real-time RT-PCR analysis. Total RNA was isolated from unelicited normal and NPC2-deficient human skin fibroblasts and quantitatively converted into the single-stranded cDNA by using a High Capacity cDNA Archive kit (Applied Biosystems). The particular genes were detected using the respective TaqMan Gene Expression Assays (Applied Biosystems) by relative quantitation employing β-glucuronidase as the reference gene. Data were normalized to their respective controls and are shown as the mean ± S.E. * depicts p ≤ 0.05 as compared with control (n = 3–6).