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Strong and selective up-regulation of PGE2 production in NPC2-null fibroblasts as measured by LC/MS/MS. Lipid analytes isolated from the conditioned media of near confluent (∼90%), unelicited normal, and NPC2-null cells were detected by tandem mass spectrometry. Data shown are the mean ± S.E. * depicts p ≤ 0.05 as compared with control (n = 4). The arrow points toward strongly and selectively up-regulated PGE2 lipid analyte.
