FIGURE 3.

Comparison of binding, insertion of the N-terminal region, and oligomerization by stopped-flow fluorescence measurements. A, a pore-forming mechanism of EqtII and different steps that were studied. Approximate positions of residues at positions 18 and 179 are presented by blue and red labels, respectively. B, fluorescence traces of different probes as denoted. The proteins that were used in each experiment are noted. The concentration of lipids was 10 μm. DOPC/SM (1:1) LUV in 140 mm NaCl, 20 mm Tris-HCl, 1 mm EDTA, pH 8.5, were used. Protein concentration was 100 nm to achieve an L/T ratio of 100. A minimum of four single injections were averaged to obtain a single fluorescent trace (gray). Residuals of the measured data to an exponential fit are shown below the traces. The red trace shows fit to a mono- or biexponential equation (as reported under “Results”).