TABLE 1.
Properties of NBD steady-state fluorescence spectra presented in Fig. 2
FL and FS represent fluorescence intensity measured at 538 nm in the presence of vesicles and in solution at a lipid/toxin ratio of 100 (for LUV) and 80 (for ghosts). n = 2–4, average ± S.D.
| EqtIIV8C,I18C,K69C-NBD-RED |
EqtIIV8C,I18C,K69C-NBD-OX |
|||
|---|---|---|---|---|
| λmax | FL/FS | λmax | FL/FS | |
| nm | nm | |||
| Solution | 539 ± 1 | 536 ± 1 | 2.8 ± 0.9a | |
| DOPC | 539 ± 2 | 1.0 ± 0.1 | 538 ± 3 | 0.90 ± 0.03 |
| DOPC/SM (1:1) | 530 ± 1 | 3.20 ± 0.04 | 535 ± 1 | 1.1 ± 0.1 |
| Ghosts | 531 ± 1 | 2.3 ± 0.3 | 535 ± 1 | 1.0 ± 0.1 |
| DOPC/5-NO-PC/SM (3:2:5) | 534 ± 1 | 1.5 ± 0.2 | 536 ± 1 | 0.90 ± 0.04 |
a This value reports the ratio between the fluorescence of oxidized and reduced version of the protein in solution.