FIGURE 7.

Effect of piceatannol and CDDP treatment on p53 activation, XIAP down-regulation, and apoptosis in vivo. A, apoptosis, as examined by immunofluorescent TUNEL staining (yellow), showing enhanced CDDP-dependent apoptosis in the presence of piceatannol. B, effect of piceatannol on nuclear Ser(P)¹⁵-p53 localization. Images are representative of overall staining patterns present in three separate tumors. C, piceatannol enhanced CDDP-dependent XIAP (red) down-regulation. Cell nuclei were stained with DAPI (blue). Fluorescence intensity in 200 cells/replicate was quantified using the ImageJ software package. **, p < 0.01; ***, p < 0.001 of combination treated group versus respective CDDP-only treated group. CTL, control.