Skip to main content
. 2013 Jun 28;288(33):23751–23764. doi: 10.1074/jbc.M113.483289

FIGURE 4.

FIGURE 4.

Time-dependent production of Gsp and T(SH)2. The reaction mixtures contained 2.1 mm ATP and 170 nm TryS as well as 0.33 mm GSH and 8.0 mm Spd (A), 0.33 mm GSH and 1.6 mm Spd (B), and 2.0 mm GSH and 8.0 mm Spd (C). After different time points, the Gsp (filled circles) and T(SH)2 (open squares) formed were quantified by HPLC analysis as outlined under “Experimental Procedures.” The average with S.D. of three independent experiments is shown. The insets provide an enlargement of the first 2 min.