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Hydrolysis of Gsp by TryS. The Gsp hydrolysis in the in vivo-like buffer system was followed by HPLC analysis. The reaction mixtures contained 1.2 μm TryS and 0.1, 0.5, 2, and 7 mm Gsp. The amidase activity as function of the Gsp concentration was fitted to a hyperbolic kinetic. The average with S.D. of at least three independent experiments is shown.
