FIGURE 3.

Epithelial-specific p38α ablation leads to a proliferation-differentiation imbalance in the skin. A and B, the shaved back skin of WT and K-KO mice was left untreated or treated with TPA (10 μg/animal) on two consecutive days; 2 days later, skin tissue sections were prepared from untreated and TPA-treated mice and analyzed by immunostaining with Ki67-specific antibody (A) and with Lys-14- and Lys-1-specific antibodies (B). Scale bar, 100 μm. C, keratinocytes from the indicated mice were treated with calcium chloride (2 mm) to induce differentiation. Whole cell lysates were prepared at the indicated time points and analyzed by immunoblotting with antibodies against the proteins indicated on the left. d, day.