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. 2013 Jul 2;288(33):23844–23857. doi: 10.1074/jbc.M113.490086

FIGURE 1.

FIGURE 1.

Muted IL-1β and IL-18 responses were observed in naïve F. tularensis (Ft)-infected macrophages. Murine macrophages were infected with 100 m.o.i. of ΔiglC mutant of F. tularensis LVS, wild type F. tularensis LVS, F. tularensis SchuS4, and F. novicida strains. Levels of IL-1β (A) and IL-18 (B) were measured in the cell culture supernatants 24 h post-infection using IL-1β flex-set and IL-18 ELISA kit. Macrophages isolated from WT, TLR2−/−, and caspase-1−/− C57BL/6 mice were infected with 100 m.o.i. of F. tularensis LVS or F. novicida. IL-1β levels in culture supernatants were analyzed in F. tularensis LVS-infected (C) or F. novicida (D)-infected macrophages. A representative of one of the three experiments conducted with similar results is shown. The data were analyzed by ANOVA with a Tukey-Kramer post-test, and a cut-off p value of 0.05 or less was considered significant. Comparisons are shown with F. novicida. *, p < 0.05; ***, p < 0.001. The levels of IL-18 in the ΔiglC mutant, F. tularensis LVS and SchuS4 infected macrophages were below the level of detection (25 pg/ml).