FIGURE 4.

FTL_0325 of F. tularensis (Ft) represses the expression of proIL-1β. Shown are Transcript levels of NLRP3, AIM2, procaspase-1, proIL-1β, and proIL-18 in IMCs derived from C57BL/6 mice. The IMCs were infected with either the FTL_0325 mutant or F. tularensis LVS at an m.o.i. of 100 for 2 h, and transcript levels were quantitated by qRT-PCR. The results are expressed as -fold change expression and are representative of three independent experiments performed using duplicate samples. The data were analyzed by ANOVA with Tukey-Kramer post-test, and a cut-off p value of 0.05 or less was considered significant. *, p < 0.05.