FIGURE 6.

Specificity of LCA inhibition. LCA inhibition of 10 nm RFTS-containing Dnmt1 (●), catalytic domain of Dnmt3a (■), and M.SssI methyltransferase (▴) was investigated at 200 nm oligonucleotide 8006 and 0.25 mm AdoMet using the Gla I-coupled DNA methylation assay. The percent activity of each condition (5–100 μm LCA) was determined by comparison to an uninhibited DMSO containing control. Fitting the data using a Hill slope of −1 gives IC₅₀ values of 27.8 ± 1.8 μm for RFTS-containing Dnmt1, 50.5 ± 4.3 μm for Dnmt3a, and 53.6 ± 4.1 μm for M.SssI methyltransferase.