FIGURE 4.

Silver-related growth phenotypes of pic2Δ yeast strains. A, serial dilutions of BY4741 and pic2Δ strains grown in rich medium with a fermentable carbon source (YPD medium) or with a non-fermentable carbon source (YPLG medium) in the presence of the cell-impermeable copper chelator BCS and increasing silver concentrations. The growth defect was reversed by addition of copper to the BCS- and silver-supplemented plates. B, Western blots for Cox2 in mitochondria isolated from cells grown in rich medium with a fermentable carbon source (upper panel) or in identical medium supplemented with 150 μm silver (lower panel). Porin served as an internal loading control.