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Phosphorylation of recombinant P450c17 by p38α is reversed by PP2A. A, P450c17 was phosphorylated by p38α and [γ-32P]ATP (lane 1) followed by incubation with either 1 (lane 2) or 10 units (lane 3) of PP2A and analyzed by autoradiography. Arrow, phospho-P450c17. B, 17,20 lyase activity of P450c17 phosphorylation by p38α and dephosphorylation by PP2A. ori, origin.
