TABLE 1.
Type of assay | Sequences (5′ to 3′) |
---|---|
shRNA | |
MAPK14 (nt 942–960 in NCBI Reference Sequence NM_001315.2) | GTAATCTAGCTGTGAATGA |
MAPK11 (nt 815–833 in NCBI Reference Sequence NM_002751.5) | GAACACGCCCGGACATATA |
Scrambled | ACATTGAAGCGAAGAATAA |
Site-directed mutagenesis | |
T341A | GTGGGTTTCAGCCGCGCACCAACTATCAGTGAC |
T341D | GTGGGTTTCAGCCGCGACCCAACTATCAGTGAC |
T341E | GTGGGTTTCAGCCGCGAACCAACTATCAGTGAC |
S427A | GGGACCCAGCTCATCGCACCGTCAGTAAGCTAT |
S427D | GGGACCCAGCTCATCGACCCGTCAGTAAGCTAT |
S427E | GGGACCCAGCTCATCGAACCGTCAGTAAGCTAT |