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. 2013 Jul 8;288(33):23903–23913. doi: 10.1074/jbc.M113.460048

TABLE 1.

Oligonucleotide sequences

nt, nucleotides.

Type of assay Sequences (5′ to 3′)
shRNA
    MAPK14 (nt 942–960 in NCBI Reference Sequence NM_001315.2) GTAATCTAGCTGTGAATGA
    MAPK11 (nt 815–833 in NCBI Reference Sequence NM_002751.5) GAACACGCCCGGACATATA
    Scrambled ACATTGAAGCGAAGAATAA
Site-directed mutagenesis
    T341A GTGGGTTTCAGCCGCGCACCAACTATCAGTGAC
    T341D GTGGGTTTCAGCCGCGACCCAACTATCAGTGAC
    T341E GTGGGTTTCAGCCGCGAACCAACTATCAGTGAC
    S427A GGGACCCAGCTCATCGCACCGTCAGTAAGCTAT
    S427D GGGACCCAGCTCATCGACCCGTCAGTAAGCTAT
    S427E GGGACCCAGCTCATCGAACCGTCAGTAAGCTAT