FIGURE 7.

Effect of Hif2α and/or Sp1 overexpression on Atp7a promoter activity. The basal Atp7a promoter construct (−224/+88) was co-transfected along with Hif2α and/or Sp1 expression plasmids into IEC-6 cells. Subsequent luciferase assays indicated promoter activity, which is shown in relation to promoter activity in the absence of Sp1 or Hif2α overexpression (Ctrl). Each bar represents the mean ± S.D. Different letters above each bar (a, b, c, and d) indicate significant differences (p < 0.05; one-way analysis of variance; n = 3–4).