FIGURE 4.

Structural basis for Rab1 recognition by LepB. A, shown is the overall view of the LepB catalytic core in complex with Rab1-GDP and AlF₃. CTD, C-terminal domain; NTD, N-terminal domain. B, shown is the non-polar interface between LepB and the switch/interswitch regions of Rab1. LepB is rendered as spheres beneath a semi-transparent surface with carbon, nitrogen, and oxygen atoms colored light slate, blue, and red, respectively. Rab1 is rendered as tubes and colored as in panel A. C, shown is are polar interactions between LepB and Rab1, defined using a 3.4 Å distance cutoff with appropriate stereochemistry. D, shown is the location of AMPylated (Tyr-80) and phosphocholinated (Ser-79) residues.