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. 2013 Jul 9;288(33):24104–24115. doi: 10.1074/jbc.M113.455832

FIGURE 2.

FIGURE 2.

Calcineurin modulates NFI-dependent promoter activity. A, U251 cells were transfected with pCAT/GFAP, pCAT/GFAP G-br1*, pCAT/GFAP G-br2*, pCAT/GFAP G-br3*, or pCAT/GFAP G-br1*/2*/3* (NFI sites mutated) and treated with 1 μm CsA or DMSO (control) for 24 h. Acetylated [14C]chloramphenicol was measured in counts/min from equal aliquots of cell lysates using a scintillation counter. The fold increases in CAT activity are relative to U251 treated with DMSO and transfected with pCAT/GFAP. The results are an average of four (pCAT/GFAP G-br1*, pCAT/GFAP G-br2*, pCAT/GFAP G-br3*, and pCAT/GFAP G-br1*/2*/3* constructs) to six (pCAT/GFAP construct) independent experiments with standard deviation indicated by error bars. B and C, U251 (B) and U87 (C) cells were co-transfected with pCAT/GFAP or pCAT/GFAP G-br1*/2*/3* and HA-DDXI (control), catalytically inactive (CNA-IN), or constitutively active (CNA-CA) HA-tagged calcineurin. The fold increases in CAT activity are relative to U251 pCAT/GFAP as follows: HA-DDXI (control) (B) or U87 pCAT/GFAP HA-DDX1 (control) (C). The results are an average of four independent experiments with standard deviation indicated by error bars. Statistical significance was determined using unpaired t test. *, denotes p < 0.01; **, denotes p < 0.001.