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mAb21 recognition of FimH in pili with and without ligand mannose in ELISA assay. mAb21 recognition in the absence of mannose reflects whether each variant is high affinity in its native state. mAb21 recognition in the presence of mannose measures the ability of each variant to change into the high affinity state upon binding ligand. Error bars represent propagation of error for S.D. of sample minus that of blank.
