FIGURE 6.

Gas chromatography-mass spectrometry chromatograms of yeast strain H1246 expressing LuPDAT1 and LuPDAT2 in the presence of ALA. The recombinant yeast cells were cultivated in the presence of ALA. The yeast lipids were extracted and separated by a thin layer chromatography (TLC) plate. The compound corresponding to the upper and lower TAG bands (marked by square brackets) was scrapped separately from the TLC plate, transmethylated, and analyzed through GC/MS. The ratio of TAG within each separated band to the total amount of TAG was calculated and the values are shown as percentage on the upper left corner of each chromatograph. GC profiles showed that yeast strain H1246 expressing LuPDAT1 or LuPDAT2 has the ability to produce TAG containing only ALA (trilinolenin), when ALA is added exogenously to the cell. P1, LuPDAT1; P2, LuPDAT2.