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. 2013 Aug 16;8(8):e71033. doi: 10.1371/journal.pone.0071033

Figure 5. Immunodetection of lactotransferrin in elephant seminal plasma samples.

Figure 5

Panels A, B, and C represent several immunoblots following 1D SDS-PAGE protein separation. E1-E6 denote individual Asian elephant bulls. G1 through G11 represent seminal plasma samples obtained from ejaculates exhibiting good motility (≥65% tMOT). P1 through P17 represent seminal plasma samples obtained from ejaculates exhibiting poor motility (≤10% tMOT). M1 represents a seminal plasma sample obtained from ejaculates exhibiting moderate motility (45% tMOT). CON: liver control. Approximately 85% of the seminal plasma samples from ejaculates exhibiting good motility were positive for the presence of lactotransferrin. Conversely, lactotransferrin was undetected in over 90% of seminal plasma samples from ejaculates exhibiting poor motility.