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. 2013 Aug 16;8(8):e71040. doi: 10.1371/journal.pone.0071040

Table 2. Primers and PCR programs for HVEM PCR-RFLP genotyping.

SNP primer restriction enzyme PCR products length Length of digested fragments
rs2281852 F: 5′-CCTACCTGCCTCTGCCATTG-3′ DdeI 186bp A:101+85bp
R: 5′-AGGGCTTCGTTGATGGGAG-3′ C: 186bp
rs1886730 F: 5′-TCCCACAGATCTCTTCCC-3′ BsmFI 678bp C: 263+226+97+50+42bp
R: 5′-CTGGGAACTGGAACTCTGC-3′ T: 489+97+50+42bp
rs2234163 F: 5′-TTGGCCTGTGGATGCTGTC-3′ HpyCH4III 245bp A: 129+116bp
R: 5′-CGCTTACCTCCCTTCTGCAC-3′ G: 245bp
rs11573979 F: 5′-CCTACCTGCCTCTGCCATTG-3′ NlaIII 292bp T: 220+47+25bp
R: 5′-AAACGAGGTGCCCAGAGGTAC-3′ C: 267+25bp
rs2234165 F: 5′-CAGACCAAGTAAGTGAACCC-3′ BfaI 221bp A: 129+92bp
R: 5′-TCTGATGAGGCTTTGTCTGGG-3′ G: 221bp
rs2234167 F: 5′-ACCGCTGTGAGACCATTG-3′ FokI 270bp A: 172+98bp
R: 5′-GGGTTCTTTCCTGAGCTAC-3′ G: 270bp