Figure 4. Microglia/monocyte accumulation precedes appearance of non-myeloid cell proliferation.

Iba1 and Ki67 dual-staining on RPE/choroid whole-mounts during the time course of CNV development. (A) Six hours post laser induction, neither macrophages nor cell proliferation are detected at lesions. (B) On day 1, accumulating Iba1⁺ cells are Ki67-negative and no proliferating cells are observed. By day 2 (C) and 4 (D), majority of macrophages are not co-localised with Ki67 immuno-reactivity, while abundant Iba1^-Ki67⁺ cells are seen at lesions. Between day 7 (E) and 14 (F), both Iba1⁺ and Ki67⁺ cell numbers subside. Images are representative of at least 12 lesions for each time point. Smaller images display separate stains for Iba1 and Ki67, respectively; bigger images are two channels merged.