Figure 6.

Differential effects on recognition of homology resulting from A⋅T transversion mismatches in “AT-rich” vs. “GC-rich” DNA. Two, four, or six mismatches at the sites of A⋅T base pairs were created by transversions in the duplex substrates, G16 (16% GC) and G37 (37% GC) (see Materials and Methods). A and B show the original data on the effects of increasing number of mismatches. In C, the yield of these reactions at 30 min was plotted against the number of mismatches. The yield at 30 min with no mismatched substrate was taken as 100%.