Figure 1. Scheme of serial hepatitis C virus passages performed in the present study.

The initial clonal population HCVcc (filled square) was prepared by transcription of plasmid Jc1 FLAG (p7-nsGluc2A) (RNA HCVcc) and electroporation into Lunet cells, as described in Materials and Methods. The amplified virus HCVp0 was subjected to serial passages in the presence of different drugs or drug combinations, as indicated; uncloned populations are depicted as empty circles. Infections with the replication-negative HCV mutant GNN were carried out in parallel (not shown in this scheme). The multiplicity of infection (MOI), drug concentrations, and the various analyses performed with different HCV populations are detailed in the corresponding experiments.