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. 2013 Aug 16;8(8):e71646. doi: 10.1371/journal.pone.0071646

Figure 2. STAT3 and STAT5b protein expression.

Figure 2

(A) Western blot analysis of the soluble (sup) and insoluble (ppt) fractions of E. coli expressing STAT5b.The proteins (5 µg) were analyzed by SDS-PAGE, and then probed with an antibody. The arrows indicate the expressed proteins. (B) CBB staining of the STAT3(136–705) and STAT5b(136–703) proteins. The purified soluble proteins (0.5 µg) were analyzed by SDS-PAGE. (C) Western blot analysis of the STAT3(136–705) and STAT5b(136–703) proteins. The blotted proteins were detected with an antibody.