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. 2013 Aug 16;8(8):e71534. doi: 10.1371/journal.pone.0071534

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© 2013 Muramatsu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Appropriately diluted viruses were mixed at indicated dilutions with the serum (A), TW (B), or NW (C) sample of mice immunized with H9N2 intranasally (i.n.) or subcutaneously (s.c.). Neutralizing activities were evaluated by counting the number of plaques formed on MDCK cells.