A: Survival time for the cardiac allografts. BALB/c derived cardiac allografts were implanted into C57BL/6J mice at the cervical site using the traditional microsuture technique and the two-stitch sleeve technique, respectively. For re-transplantation, the recipient mice were first transplanted with a cardiac allograft at the abdominal site, and 3 weeks later a second cardiac allograft was implanted at the cervical site using the two-stitch sleeve technique. B: Comparison for the time of arterial anastomosis between the sleeve technique and traditional technique. POD, post operative day; MST, median survival time. **, p < 0.01.