Figure 5.

MCP-1 secretion is sensitive to changes in the intracellular but not extracellular Ca²⁺ concentration: Lowering of the extracellular Ca²⁺ concentration did not affect MCP-1 secretion from control or TREK-1 deficient A549 cells after 6 hours of TNF-α stimulation (A). Inhibition of intracellular Ca²⁺ reuptake mechanisms by thapsigargin decreased MCP-1 secretion to a similar degree in control and TREK-1 deficient cells after 6 hours of TNF-α stimulation (B). *comparison of thapsigargin-exposed and unexposed TNF-α stimulated control cells; #comparison of thapsigargin-exposed and unexposed TNF-α stimulated TREK-1 deficient cells; n = 3, p ≤ 0.05.