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Methylation of ANXA6 promoter CGI mediates ANXA6 downregulation. A: ANXA6 contains a typical CpG Island (CGI) in its promoter region. The number indicates the location of CGI from the transcription start site. One vertical black line indicate one CpG site. B: Expression of ANXA6 before and after Aza treatment were determined by real-time RT-PCR as in Figure 1B. Methylation of ANXA6 promoter in GES-1 and gastric cancer cells were determined by MSP (C) and BGS (D).
