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. 2013 Aug 16;8(8):e71205. doi: 10.1371/journal.pone.0071205

Figure 1. qRT-PCR of selected genes that were differentially expressed based on suppression subtractive hybridization.

Figure 1

Relative quantification of mRNA expression (fold change) of (A) pyruvate kinase (pk, JZ347493), (B) glutamine synthetase (gs, JZ347462), (C) fumarate hydratase (fh, JZ347458), (D) prolactin (prl, JZ347487), (E) phosphofructokinase (pfk, JZ347479), (F) Na+/K+-ATPase α2 (nkaα2, JZ347474), (G) pyruvate kinase (pk, JZ347493), (H) prolactin (prl, JZ347487) and (I) ferritin heavy chain (fth, JZ347374), using β-actin as the reference gene, in the brain of Protopterus annectens aestivated for 6 days (d) (A–F) or 6 months (G–I) in air with reference to the those of fish kept in freshwater as control. Results represent mean+S.E.M. (N = 6). *Significantly different from the corresponding freshwater control (P<0.05).