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. 2013 Aug 16;8(8):e71205. doi: 10.1371/journal.pone.0071205

Table 2. Known transcripts found in the forward library (up-regulation) obtained by suppression subtractive hybridization PCR from the brain of Protopterus annectens aestivated for 6 days in air with fish kept in freshwater as the reference for comparison.

Group and Gene P. annectens accession no. Biological processes E-value No of clones
Apoptosis
Plasminogen activator inhibitor 1 RNA-binding protein JZ347480 Regulation of anti-apoptosis 3.00E-07 1
Carbohydrate metabolism
Enolase JZ347451 Glycolysis 4.00E-31 1
Fructose-bisphosphate aldolase C JZ347457 Glycolysis 4.00E-91 3
Fumarate hydratase JZ347458 TCA cycle 5.00E-30 2
Pyruvate kinase JZ347493 Glycolysis 9.00E-35 1
Cell cycle and proliferation
BRCA2 and CDKN1A interacting protein JZ347437 Cell cycle and DNA repair 2.00E-57 1
Growth hormone precursor JZ347464 Positive regulation of growth 0 2
RAN, member RAS oncogene family JZ347497 Cell cycle 3.00E-173 1
Secreted acidic cysteine rich glycoprotein JZ347521 Regulation of cell proliferation 1.00E-65 4
Lipoprotein, fatty acid and cholesterol homeostasis and transport
c11orf2 homolog JZ347439 Lipid transport 8.00E-56 1
Ion binding and transport
ADP/ATP translocase 2 putative JZ347428 Membrane transport 8.00E-50 5
Mitochondrial ATP synthase beta subunit JZ347472 ATP synthesis 9.00E-35 5
Sec61 beta subunit JZ347520 Membrane transport 1.00E-64 2
Solute carrier family 20, member 1b JZ347524 Phosphate transport 3.00E-17 1
Solute carrier family 25 alpha, member 5 JZ347526 Membrane transport 1.00E-51 1
Iron metabolism and transport
Apoferritin higher subunit JZ347429 Iron binding 2.00E-88 1
Nitrogen metabolism
Glutamine synthetase JZ347462 Glutamine biosynthetic process 2.00E-23 2
Nucleic acid binding and transcription
Brain abundant, membrane attached signal protein 1 JZ347436 Negative regulation of gene-specific transcription 8.00E-18 2
Breast carcinoma amplified sequence 2 JZ347438 RNA splicing 1.00E-83 2
Ctr9, Paf1/RNA polymerase II complex component JZ347445 Histone monoubiquitination 0 1
DEAH (Asp-Glu-Ala-His) box polypeptide 15 JZ347449 RNA splicing 6.00E-07 1
H3 histone, family 3B JZ347379 Nucleosome assembly 3.00E-74 1
High mobility group protein-1 JZ347466 Positive regulation of transcription 9.00E-16 1
Histone H2A.Z putative mRNA JZ347467 Nucleosome assembly 3.00E-97 3
p68 RNA helicase JZ347477 ATP dependent helicase activity 7.00E-06 4
Poly(A) binding protein, cytoplasmic 1 JZ347481 RNA splicing 2.00E-54 2
Polymerase (RNA) II (DNA directed) polypeptide J JZ347482 Transcription 2.00E-36 3
Small nuclear ribonucleoprotein E JZ347523 RNA splicing 2.00E-64 2
SRA stem-loop-interacting RNA-binding protein, mitochondrial precursor JZ347527 Regulation of transcription 8.00E-38 1
Tripartite motif protein 28 JZ347537 Regulation of transcription 5.00E-19 1
Y box binding protein 1 isoform 2 JZ347547 mRNA splicing 6.00E-61 2
Protein degradation
Proteasome (prosome macropain) subunit alpha type 4 JZ347489 Proteolysis 5.00E-95 1
Protein synthesis, transport and folding
40S ribosomal protein S2 JZ347424 Translation 3.00E-65 2
40S ribosomal protein S30 JZ347425 Translation elongation 8.00E-37 4
Amyloid beta (A4) precursor protein JZ347354 Protein transport 6.00E-48 5
Eukaryotic translation elongation factor 1 alpha 2 JZ347371 Translation 4.00E-25 40
Eukaryotic translation elongation factor 1 beta 2 JZ347452 Translation 9.00E-54 1
Eukaryotic translation initiation factor 3, subunit 3 gamma JZ347454 Translation 2.00E-28 1
Lipocalin JZ347384 Protein transport 1.00E-23 28
Prefoldin subunit 2 JZ347483 Protein binding 1.00E-31 1
RAB36, member RAS oncogene family JZ347495 Protein transport 5.00E-11 1
Ribosomal protein L12 JZ347505 Translation 7.00E-74 5
Ribosomal protein L3 fragment 1 JZ347500 Translation 2.00E-35 2
Ribosomal protein L35 JZ347509 Translation 7.00E-41 2
Ribosomal protein L41 JZ347510 Translation 3.00E-21 1
Ribosomal protein L4-like JZ347502 Translation 5.00E-133 2
Ribosomal protein L5 JZ347503 Ribosome assembly 0 1
Ribosomal protein L7a-like JZ347392 Ribosome biogenesis 9.00E-78 8
Ribosomal protein S12 JZ347516 Translation 3.00E-36 2
Ribosomal protein S27a JZ347519 Translation 5.00E-97 3
Ribosomal protein S2e JZ347511 Translation 1.00E-75 1
Ribosomal protein S3 JZ347512 Translation 0 2
Ribosomal protein S4 JZ347514 Translation 0 8
Transmembrane protein 11 JZ347536 Protein binding 2.00E-17 2
Response to stimulus
Cold-inducible RNA-binding protein JZ347442 Response to stress 4.00E-33 3
Ganglioside-induced differentiation-associated protein 1-like 1 JZ347460 Response to retinoic acid 2.00E-29 2
Signaling
Prolactin JZ347487 Lactation, positive regulation of cell proliferation 5.00E-14 15
RAC/CDC42 exchange factor JZ347496 Regulation of Rho protein signal transduction 2.00E-39 1
RAS-like, family 11, member B JZ347498 Signal transduction 1.00E-25 2
Reticulon 1-C.1 JZ347499 Signal transduction 2.00E-04 1
Structural
Tubulin alpha-1 chain putative JZ347538 Cell structure 9.00E-170 9
Tubulin, alpha 4a JZ347416 Cell structure 0 11
Tubulin, beta JZ347540 Cell structure 7.00E-44 1
Tubulin, beta 5 JZ347541 Cell structure 5.00E-164 1
Others
ATG7 autophagy related 7 homolog JZ347431 Autophagy 2.00E-44 1
Cardiac muscle alpha actin 1 JZ347440 Unclassified 2.00E-67 1
Cell cycle associated protein 1b JZ347441 Unclassified 2.00E-12 2
Creatine kinase, mitochondrial 1 JZ347444 ATP binding 3.00E-150 1
Dynamin 1-like JZ347450 GTP catabolic process 2.00E-26 6
Fumarylacetoacetate hydrolase JZ347459 Cellular amino acid metabolic process 8.00E-10 2
Glucose regulated protein, 58 kDa JZ347461 Unclassified 1.00E-40 1
HMP19 protein JZ347468 Unclassified 6.00E-43 2
Myosin, light chain 1, alkali; skeletal, fast, JZ347473 Muscle contraction 4.00E-27 1
Nasopharyngeal epithelium specific protein 1 JZ347475 Unclassified 1.00E-46 2
Ornithine decarboxylase antizyme JZ347476 Cell differentiation 2.00E-137 3
Prolyl 4-hydroxylase, alpha polypeptide II JZ347488 Unclassified 1.00E-25 2
Quinoid dihydropteridine reductase JZ347494 Metabolic process 2.00E-19 1
Stromal cell derived factor receptor 1 JZ347529 Unclassified 1.00E-39 6
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide JZ347542 Protein targeting 1.00E-54 2
Ubiquinol-cytochrome c reductase core protein I JZ347543 Electron transport chain 8.00E-55 2