Table 3. Known transcripts found in the reverse library (down-regulation) obtained by suppression subtractive hybridization PCR from the brain of Protopterus annectens aestivated for 6 days in air with fish kept in freshwater as the reference for comparison.

Group and Gene	P. annectens accession no.	Biological processes	E-value	No of clones
Carbohydrate metabolism
Glyceraldehyde-3-phosphate dehydrogenase	JZ347463	Glycolysis	6.00E-22	1
Phosphofructokinase	JZ347479	Glycolysis	4.00E-163	7
Cell cycle and proliferation
Protein phosphatase 1 (pp1) gamma 1	JZ347490	Cell cycle	2.00E-172	11
SUMO-conjugating enzyme UBC9	JZ347531	Cell cycle	4.00E-147	5
Cell structure
Actin, gamma 1	JZ347426	Cell structure	3.00E-70	4
Transmembrane protein 2	JZ347535	Integral to membrane	1.00E-49	2
Tubulin, alpha 4a	JZ347539	Microtubule–based process	9.00E-16	1
UPF0466 protein C22orf32, mitochondrial	JZ347545	Integral to membrane	1.00E-12	3
Complement
Complement component factor H	JZ347443	Complement activation	6.00E-07	2
DNA repair and protection
Activity-dependent neuroprotector homeobox	JZ347427	Neuroprotection	2.00E-16	3
Apoptosis-inducing, TAF9-like domain 1	JZ347430	DNA repair	2.00E-16	1
Ion binding and transport
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c-3	JZ347432	ATP synthesis coupled proton transport	1.00E-41	1
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f	JZ347433	ATP synthesis coupled proton transport	6.00E-33	2
ATPase, Na+/K+ transporting, beta 1a polypeptide	JZ347434	Ion transport	1.00E-09	1
Na+/K+-ATPase alpha 2 subunit	JZ347474	Cation transport	0	3
Solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3	JZ347525	Transmembrane transport	1.00E-30	2
Voltage-dependent anion-selective channel protein 2	JZ347546	Anion transport	8.00E-132	1
Iron, copper metabolism and transport
Beta-2-globin	JZ347435	Oxygen transport	7.00E-05	2
Lipoprotein, fatty acid and cholesterol homeostasis and transport
Lipoyltransferase 1	JZ347471	Protein lipoylation	9.00E-27	3
Sulfotransferase family 4A, member 1	JZ347530	Lipid metabolic process	1.00E-72	1
Nucleic acid binding and transcription
KH domain containing, RNA binding, signal transduction associated 1b	JZ347469	RNA binding	3.00E-05	5
Protein phosphatase 1A magnesium-dependent, alpha isoform	JZ347491	Regulation of transcription	4.00E-79	1
Prothymosin, alpha	JZ347492	Transcription	4.00E-24	13
Staufen 1	JZ347528	Intracellular mRNA localization	2.00E-35	3
Transcription elongation factor B polypeptide 2	JZ347533	Regulation of transcription	6.00E-40	1
Translin	JZ347534	DNA binding	3.00E-71	1
Protein degradation
Ubiquitin conjugating enzyme E2	JZ347544	Protein degradation	2.00E-90	9
Serpin peptidase inhibitor, clade B (ovalbumin),member 1, like 3	JZ347522	Serine type endopeptidase inhibitor activity	1.00E-08	1
Protein synthesis, transport and folding
Ribosomal protein L3 fragment 2	JZ347501	Translation	2.00E-28	1
DEAD (Asp-Glu-Ala-Asp) box polypeptide 21	JZ347448	Ribosomal RNA biogenesis, editing, transport	4.00E-05	4
Eukaryotic translation elongation factor 2	JZ347453	Translation	2.00E-39	1
Eukaryotic translation initiation factor 4E-binding protein 2	JZ347455	Translation initiation	4.00E-09	9
Procollagen C-endopeptidase enhancer 2	JZ347486	Heparin/protein binding	1.00E-23	1
Ribosomal protein L22-like 1	JZ347506	Translation	4.00E-79	1
Ribosomal protein L31	JZ347507	Translation	3.00E-88	3
Ribosomal protein L34	JZ347508	Translation	6.00E-88	1
Ribosomal protein L7a-like	JZ347504	Ribosome biogenesis	2.00E-101	3
Ribosomal protein S13	JZ347517	Ribosome assembly	5.00E-105	1
Ribosomal protein S20	JZ347518	Translation	6.00E-85	1
Ribosomal protein S3A	JZ347513	Translation	5.00E-146	1
Ribosomal protein S9	JZ347515	Translation	4.00E-122	10
Response to stimulus
Cyclic AMP-regulated phosphoprotein, 21 kD	JZ347446	Cellular response to heat	4.00E-22	9
FK506 binding protein 1B, 12.6 kDa	JZ347456	Regulation of heart rate, response to glucose stimulus	7.00E-68	4
Signaling
Guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O	JZ347465	G-protein coupled receptor protein signaling pathway	2.00E-04	1
Phosphatidylethanolamine binding protein	JZ347478	Regulation of mitosis and signaling	4.00E-52	1
Others
Cytochrome c oxidase subunit VIa polypeptide 1	JZ347447	Oxidation reduction	1.00E-27	14
KH domain-containing transcription factor B3	JZ347470	Unclassified	4.00E-10	3
Prepromesotocin	JZ347484	Unclassified	5.00E-32	1
Preprosomatostatin 2	JZ347485	Regulation of cell migration	3.00E-164	5
Telomere associated repeat sequence	JZ347532	Unclassified	2.00E-20	1