Table 5. Known transcripts found in the reverse library (down-regulation) obtained by suppression subtractive hybridization PCR from the brain of Protopterus annectens aestivated for 6 months in air with fish kept in freshwater as the reference for comparison.

Group and Gene	P. annectens accession no.	Biological processes	E-value	No of clones
Cell structure
Beta-tubulin	JZ347417	Cell structure	2.00E-67	1
Myristoylated alanine-rich protein kinase C substrate	JZ347386	Cell structure	2.00E-10	3
Ion binding and transport
ATPase, H+ transporting, V0 subunit C	JZ347356	ATP synthesis coupledproton transport	4.00E-25	4
Basic leucine zipper and W2 domains 1	JZ347357	Regulation of transcription	2.00E-05	1
Iron metabolism and transport
Ferritin heavy chain	JZ347374	Cellular iron ion homeostasis,iron ion transport	2.00E-75	1
Protein synthesis, transport and folding
Coatomer protein complex, subunit gamma 2	JZ347361	Intracellular protein transport,vesicle-mediated transport	7.00E-23	104
DnaJ (Hsp40) homolog, subfamily A, member 1	JZ347365	Protein folding	4.00E-33	1
Elongation factor-1, delta, b	JZ347368	Translation elongation	4.00E-63	1
Ribosomal protein L19 fragment 2	JZ347398	Translation	2.00E-82	3
Ribosomal protein L36a	JZ347402	Translation	2.00E-62	2
Ribosomal protein L38	JZ347403	Translation	1.00E-32	1
Ribosomal protein L8	JZ347393	Translation	6.00E-112	3
Signaling
Prolactin	JZ347487	lactation, positive regulation ofcell proliferation	1.00E-20	43
Others
Gephyrin	JZ347376	Establishment of synaptic specificityat neuromuscular junction	0	1
HN1-like protein	JZ347383	Unclassified	2.00E-05	4
KH domain-containing transcription factor B3	JZ347470	Unclassified	4.00E-08	146
NADH dehydrogenase 1 alpha subcomplex subunit 13	JZ347387	Electron transport chain	4.00E-45	3
Peptidyl-glycine alpha-amidating monooxygenase A precursor	JZ347388	Peptide metabolic process,oxidation reduction	3.00E-28	1