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. 2013 Aug 16;8(8):e71282. doi: 10.1371/journal.pone.0071282

Figure 4. PTTG1 promoter activity is suppressed during PMA-induced THP1 or HL-60 cell differentiation.

Figure 4

(A) Schematic diagram of a construct of PTTG1 promoter-luciferase reporter. Position +1 was assigned to the nucleotide at the transcription start site. The control pGL3-basic vector or the PTTG1-P1 construct was co-transfected with the Renilla internal control vector into THP1 or HL-60 cells. Twenty-four hours after transfection, cells were treated with vehicle (0.1% DMSO) or PMA (200 nM) for 24 h. Cells were then harvested for the luciferase reporter assay. PTTG1 promoter activity was detected in PMA-primed THP1 (B) and HL-60 (C) cells. The intensities of the luciferase reactions measured in the lysates of the transfected cells were normalized to their Renilla luciferase control activity. Data represent the mean ± SD from three independent experiments. ** p<0.01 represents significant differences compared with the vehicle-treated cells.