Figure 5. Characterization of a PMA-responsive regulatory element within the PTTG1 promoter.

(A) Schematic diagram of the serial deleted constructs of PTTG1 promoter luciferase reporters. (B) The reporter constructs were transiently co-transfected with the Renilla luciferase control vector into THP1 cells. Twenty-four hours after transfection, cells were treated with vehicle (0.1% DMSO) or PMA (200 nM) for 24 h. Cells were then harvested for the luciferase reporter assay. The intensities of the luciferase reactions measured in the lysates of the transfected cells were normalized to their Renilla luciferase control activity. Data represent the mean ± SD from three independent experiments. ** p<0.01 represents significant differences compared with the vehicle-treated cells.