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. 2013 Aug 16;8(8):e71282. doi: 10.1371/journal.pone.0071282

Figure 8. The effect of KLF6 knockdown on PTTG1 transcription during PMA-induced cell differentiation.

Figure 8

(A) Knockdown of KLF6 expression was performed using a specific lentiviral vector encoding KLF6 shRNA, and stable clones were selected with puromycin (2 µg/ml) for 2 weeks. Western blot of nuclear extracts was performed to examine the knockdown efficiency of shKLF6-THP1 and shKLF6-HL-60 cells compared with cells transfected with shLacZ lentiviral vector. Cells with stable KLF6 knockdown were treated with PMA (200 nM) for 72 h. Total cellular RNA was extracted and PTTG1 mRNA expression was determined by qRT-PCR in (B) THP1 and (C) HL-60 cells. Data represent the mean ± SD from three independent experiments. **p<0.01 represents significant differences compared with PMA non-treated cells. ## p<0.01 represents significant differences compared with the PMA-treated shLacZ knockdown control group.