Table 2. Nature of liposome-specific domains and membrane-specific domain of SecA ^a .

	Membrane (%)			Liposomes (%)			Liposomes+N39 (%)
	TK	Na2CO3	Heparin	TK	Na2CO3	Heparin	TK	Na2CO3	Heparin
SecA	100	123±7.2	68±12	100	130±9.5	81±33	100	115±12	95±34
68kDa	100	113±10	92±23	100	128±7.8	88±31	100	110±25	75±23
48kDa	100	36±4	102±19	100	42±8.3	100±33	100	50±20	106±34
39kDa	100	101±13	100±28	100	130±25	80±36	100	100±17	68±9.3

^aReaction mixtures with 1 µg [³⁵S]SecA were incubated and treated as in Fig. 5.C. The membranes, liposomes and liposomes with N39 (20 µg/ml) and were recovered by centrifugation and re-suspended in 100 μl of TK buffer, 10 mg/ml heparin or 0.1 M Na₂CO₃ (pH 11) respectively. After incubation on ice for 30 min, the liposomes and membranes were recovered by centrifugation and were analyzed by SDS-PAGE and autoradiography. The quantities of remaining SecA fragments in liposomes and membranes were determined by Quantity One software, using these in TK buffer as 100%. All experiments were carried out for 3-5 times.