Table 1.
Summary of eight samples sequenced with 100 base reads after targeted enrichment of STR loci
| Sample Name | NA19240 | NA19240 | NA19238 | NA19239 | NA18501 | NA18502 | CHM1 | CHM2 |
|---|---|---|---|---|---|---|---|---|
| Number of PCR cycles used during library preparation | 0 | 13 | 3 | 0 | 2 | 3 | 3 | 13 |
| Total number of reads Produced, millions | 16.1 | 81.4 | 18.6 | 33.8 | 15.2 | 13.2 | 11.6 | 11.9 |
| Number of STR loci genotyped (% of target STRs with length ≤94bp) | 3,178 (50.9%) | 3,648 (58.4%) | 3,295 (52.8%) | 3,403 (54.5%) | 3,227 (51.7%) | 3,197 (51.2%) | 3,121 (50.0%) | 3,121 (50.0%) |
| Total number of on target reads, millions (STR±200bp) (% of total reads) | 6.52 (40.5%) | 30.95 (38.0%) | 7.31 (39.3%) | 12.59 (37.2%) | 5.65 (37.2%) | 5.00 (37.9%) | 4.37 (37.7% | 4.95 (41.6%) |
| Total number of informative reads that span STRs (% of total reads) | 363,534 (2.3%) | 1,766,728 (2.2%) | 425,012 (2.3%) | 691,640 (2.0%) | 325,541 (2.1%) | 294,007 (2.2%) | 249,844 (2.2%) | 271,351 (2.3%) |
| Percentage of STRs with ≥1 informative read | 54% | 67% | 56% | 58% | 55% | 55% | 53% | 54% |
| Percentage of STRs with ≥5 informative reads | 50% | 64% | 53% | 54% | 51% | 51% | 48% | 49% |
| Percentage of STRs with ≥10 informative reads | 47% | 61% | 50% | 51% | 47% | 47% | 45% | 45% |
| Percentage of STRs with ≥100 informative reads | 20% | 46% | 23% | 31% | 18% | 17% | 14% | 16% |