Figure 2. Osmotic stress-induced elevations in steady-state levels of the Group 3 LEA protein genes, Phypa_166566 and Phypa_211998 in Physcomitrella patens protonemata. Elevations in steady-state levels of Phypa_166566 induced by (A) 0.3 M mannitol and (B) 0.4 M mannitol (three independent biological replicates represented by closed symbols). Elevations in steady-state levels of Phypa_211998 induced by (C) 0.3 M mannitol and (D) 0.4 M mannitol (three independent biological replicates represented by closed symbols). Relative expression levels were determined by normalization of transcript levels of Phypa_166566 and Phypa_211998 with the geometric mean of stably expressed 18s rRNA, actin (Phypa_109052) and tubulin (Phypa_170860).