Table 3. The endophytic fungal isolates from three species of seagrasses with strong antimicrobial activity.
|
Seagrass
|
a)
Crude extract (Fungal identification) |
Accession no.
|
b)
MIC /MBC or MFC values (µg/ml) |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| c) SA | MRSA | CA1 | CA2 | CN1 | CN2 | MG | ||||||||||||
| Cymodocea serrulata | ||||||||||||||||||
| PSU-ES26CH (Hypocreales sp.) | JN116602 | 8/128 | 8/128 | 16/>200 | 8/128 | 2/16 | ||||||||||||
| Halophila ovalis | ||||||||||||||||||
| PSU-ES8CE ( Trichoderma sp.)d | 64/>200 | 128/>200 | 2/>200 | |||||||||||||||
| PSU-ES38CE ( Trichoderma sp.)d | 64/>200 | 1/>200 | ||||||||||||||||
| Thalassia hemprichii | ||||||||||||||||||
| PSU-ES43CH ( Penicillium sp.) | JN116612 | 8/16 | 32/64 | |||||||||||||||
| PSU-ES73CE ( Fusarium sp.) | JQ733502 | 8/>200 | 8/>200 | ¼ | 4/8 | |||||||||||||
| PSU-ES172BE ( Stephanonectria sp.) | JN116689 | 8/>200 | ||||||||||||||||
| PSU-ES172CE ( Stephanonectria sp.) | JN116689 | 8/>200 | ||||||||||||||||
| PSU-ES190CH (mycelia sterilia) | 4/>200 | 32/64 | ||||||||||||||||
| Antibiotic drugs | ||||||||||||||||||
| Vancomycin | 0.5/1 | 1/2 | ||||||||||||||||
| Amphotericin B | 0.125/0.25 | 0.125/0.5 | 0.125/1 | 0.25/2 | ||||||||||||||
| Miconazole | 1/32 | |||||||||||||||||
a)CH, hexane extract from fungal cell; BE, ethyl acetate extract from culture broth; CE, ethyl acetate extract from fungal cell
b)MIC, minimum inhibitory concentration; MBC minimum bactericidal concentration; MFC, minimum fungicidal concentration
c)SA, Staphylococcus aureus ATCC 25923; CA1, Candida albicans ATCC 90028; CA2, C. albicans NCPF 3153; CN1, Cryptococcus neoformans ATCC 90112 (flucytosine-sensitive); CN2, C. neoformans ATCC 90113 (flucytosine-resistant); MG, Microsporum gypseum clinical isolate; PM, Penicillium marneffei clinical isolate
d)Identified by morphological characteristics