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. 2001 Jul 17;98(15):8447–8453. doi: 10.1073/pnas.121009898

Figure 4.

Figure 4

Rad54 protein stimulates the rate of hDNA extension in Rad51 protein-mediated DNA strand exchange. (A) Graphic representation of the effect of Rad54 protein on hDNA extension derived from the data shown in Fig. 3 A–C. Plotted are the time points when 50% of the joint molecules have passed the physical distance defined by the three restriction sites. Reactions contained S. cerevisiae Rad51 protein and Rpa as well as storage buffer (Rad51 control; ○), Rad54 (●), or Rad54-K341R (*). (B) Intervals in DNA strand exchange. ΦX174 dsDNA linearized with PstI is represented on top and has a total length of 5,386 bp. The direction refers to the virion DNA (plus strand). The restriction sites used for this analysis are indicated. Rates for hDNA extension could be calculated for intervals I–VI. (C) Rad54 protein stimulates the rate of hDNA extension. The intervals are illustrated in B. The numbers in parentheses represent the fold stimulation in comparison to control reactions containing Rad51 protein to which protein storage buffer was added.